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脊髓损伤后小鼠泌尿膀胱分子分析的单细胞分离方法

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury

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脊髓损伤后小鼠泌尿膀胱分子分析的单细胞分离方法

DOI:

10.3791/61455-v

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09:50 min

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June 17, 2020

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Hussein Atta*^1,2, Ali Hashemi Gheinani*^1,2, Amanda Wacker, Yaser Heshmati^4,5, Alex Bigger-Allen⁶, George Lambrinos², Yao Gao⁷, Diane R. Bielenberg⁷, Rosalyn M. Adam²

¹Department of Urology,Boston Children’s Hospital, ²Department of Surgery,Harvard Medical School, ³Division of Hematology/Oncology,Harvard Medical School Boston, ⁴Dana-Farber Cancer Institute, ⁵Broad Institute, ⁶Biological Biomedical Sciences Program, Division of Medical Sciences,Harvard Medical School, ⁷Vascular Biology Program,Boston Children’s Hospital

Chapters

00:04Introduction
00:57Mouse Preparation
01:48Spinal Cord Injury (SCI)
03:17Tissue Perfusion and Procurement
04:34Cell Dissociation and Single Cell Suspension Preparation
05:59Cell Staining
06:44Flow Cytometry Analysis
07:43Results: Representative Mouse Bladder Collagen Expression and Immune Cell Population Analyses
08:54Conclusion

Summary

自动翻译

此协议的目标是将优化的组织分离协议应用于脊髓损伤的鼠标模型，并验证通过流细胞学进行单细胞分析的方法。

Tags

Single Cell Dissociation Molecular Analysis Urinary Bladder Mouse Spinal Cord Injury FACS Analysis Laminectomy Spinal Cord Transection Bladder Expression

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