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Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
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June 30, 2020
DOI:
1Unidad de Hortofruticultura,Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Unidad de Producción y Sanidad Animal,Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 3Dpto Química Analítica,Escuela Politécnica Superior (Universidad de Zaragoza), 4Instituto Agroalimentario de Aragón – IA2 (CITA-Universidad de Zaragoza)
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We present a fast and reliable method to quantify vitamin C in Lactuca spp. using UPLC-UV, potentially transferable to other plants. The key steps are the sample preparation and vitamin C extraction under stable conditions, the reduction of dehydroascorbic acid to ascorbic acid and the optimization of the chromatographic procedure.
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Medina-Lozano, I., Bertolín, J. R., Zufiaurre, R., Díaz, A. Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.). J. Vis. Exp. (160), e61440, doi:10.3791/61440 (2020).
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