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Modellering Hepatitis B Virus Infektion i ikke-hepatisk 293T-NE-3NRs celler
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免疫与感染
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JoVE 杂志 免疫与感染
Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
DOI:

09:02 min

June 05, 2020

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Chapters

  • 00:04Introduction
  • 01:36HepG2.2.15 Cell Culture
  • 03:04Detection of HBV DNA in Supernatant Concentrate
  • 04:23In Vitro Infection of HepG2-NE and 293T-NE-3NRs Cells
  • 05:27HepG2.2.15 Co-culture with HepG2-NE / 293T-NE-3NRs / 293T-NE
  • 06:41Results: HBcAg Expression in 293T-NE, 293T-NE-3NRs and HepG2-NE
  • 07:47Conclusion

Summary

自动翻译

Dette manuskript beskriver en detaljeret protokol for Hepatitis B-virus (HBV) infektion i nye manipuleret 293T celler (293T-NE-3NRs, der udtrykker menneskelige NTCP, HNF4α, RXRα og PPARα) og traditionelle leverceller (HepG2-NE, der udtrykker menneskelige NTCP).

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