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Visualisering af synaptisk degeneration hos Adult Drosophila i samarbejde med neurodegeneration
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration

Visualisering af synaptisk degeneration hos Adult Drosophila i samarbejde med neurodegeneration

DOI:
10.3791/61363-v

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06:10 min

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May 13, 2020

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1Department of Biological Sciences,Lehigh University

Chapters

  • 00:04Introduction
  • 00:41Thorax Isolation and Fixation
  • 02:12Flash Freezing and Thorax Bisection
  • 03:41Structural Staining
  • 04:08Tissue Mounting
  • 04:57Results: Representative Synaptic Integrity Assessment
  • 05:46Conclusion

Summary

自动翻译

自动翻译

Målet med denne procedure er at dissekere dorsal langsgående muskel (DLM) væv til at vurdere den strukturelle integritet DLM neuromuskulære kryds (NMJs) i neurodegenerative sygdom modeller ved hjælp af Drosophila melanogaster.

Tags

Synaptic DegenerationAdult DrosophilaNeurodegenerationNeuromuscular JunctionsALSParkinson’sHuntington’sAlzheimer’s DiseaseDissectionAnesthesiaForcepsDissection ScissorsFormaldehydeCryoprotective GlovesLiquid NitrogenFeather BladePasteur Pipette

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