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Digestione di interi occhi di topo per l'analisi citometrica a flusso multi-parametro dei fagociti mononucleari
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Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
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Digestione di interi occhi di topo per l'analisi citometrica a flusso multi-parametro dei fagociti mononucleari

4,704 Views

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09:58 min

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June 17, 2020

DOI:

10.3791/61348-v

DOI:
10.3791/61348-v

•

09:58 min
June 17, 2020

•

2 Views
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1Department of Ophthalmology, Feinberg School of Medicine,Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine,Northwestern University

Summary

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Questo protocollo fornisce un metodo per digerire interi occhi in una singola sospensione cellulare ai fini dell'analisi citometrica del flusso multi-parametro al fine di identificare specifiche popolazioni fagocitiche mononucleari oculari, inclusi monociti, microglia, macrofagi e cellule dendritiche.

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Cite this Article

Droho, S., Cuda, C. M., Lavine, J. A. Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes. J. Vis. Exp. (160), e61348, doi:10.3791/61348 (2020).

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