Journal
/
发育生物学
/
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

DOI:
10.3791/61086-v

•

08:37 min

•

October 09, 2020

•

,
1Development, Disease Models & Therapeutics Graduate Program, Department of Molecular and Cellular Biology, Department of Neuroscience, Center for Cell and Gene Therapy, Stem Cells and Regenerative Medicine Center,Baylor College of Medicine

Chapters

  • 00:04Introduction
  • 01:00Embryo Dissection
  • 01:51Embryo Dissociation and Cell Sorting
  • 03:11Size Selection
  • 06:02Results: Small RNA Libraries
  • 07:53Conclusion

Summary

自动翻译

自动翻译

We describe a technique for profiling microRNAs in early mouse embryos. This protocol overcomes the challenge of low cell input and small RNA enrichment. This assay can be used to analyze changes in miRNA expression over time in different cell lineages of the early mouse embryo.

Tags

Small RNA SequencingMicroRNAsEmbryonic DevelopmentLow Input SamplesEmbryo DissectionEmbryo StagingCell SuspensionCell SortingRNA Extraction

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

Generation of Naïve Blastoderm Explants from Zebrafish Embryos

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Read Article