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1Department of Pulmonary Diseases, Amsterdam UMC,VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group,University of Twente, 3Applied Stem Cell Technologies Group,University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC,VU University Medical Center, 5Institute of Physiology,Charité-Universitätsmedizin, 6German Heart Center
Chapters
- 00:04Introduction
- 00:59Primary Human Pulmonary Arterial Endothelial Cell (PAEC) Isolation and Culture
- 02:43Flow Chambers and PAEC Monolayer Preparation
- 04:00Washed Human Whole Blood Preparation
- 04:50Flow System Assembly
- 05:41Washed Whole Blood Perfusion
- 06:44Results: Representative Thrombus Formation Characterization
- 08:29Conclusion
Vi presenterer en in vitro vaskulær sykdom modell for å undersøke fullblods interaksjoner med pasient-avledet endotel. Dette systemet tillater studiet av trombogene egenskaper av primære endotelceller under ulike omstendigheter. Metoden er spesielt egnet til å evaluere in situ trombogenitet og antikoagulasjonsbehandling i ulike faser av koagulasjon.
