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Exon Pulando em miotubos reprogramados diretamente obtidos a partir de células derivadas da urina humana
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells

Exon Pulando em miotubos reprogramados diretamente obtidos a partir de células derivadas da urina humana

6,707 Views

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06:20 min

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May 07, 2020

DOI:

10.3791/60840-v

DOI:
10.3791/60840-v

•

06:20 min
May 07, 2020

•

2 Views
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1Department of Molecular Therapy, National Institute of Neuroscience,National Center of Neurology and Psychiatry

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Neste artigo, descrevemos um protocolo detalhado para modelagem eficiente do músculo distrofia muscular de Duchenne usando células derivadas de urina convertidas por MYOD1para avaliar a restauração de mRNA de distrofina e níveis de proteína após o salto do exon.

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Takizawa, H., Sato, M., Aoki, Y. Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells. J. Vis. Exp. (159), e60840, doi:10.3791/60840 (2020).

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