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Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation
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生物化学
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JoVE 杂志 生物化学
Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

DOI:
10.3791/60815-v

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08:22 min

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January 22, 2020

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, ,
1Department of Pharmacology,Weill Cornell Medical College

Chapters

  • 00:04Title
  • 00:32Sensor Cartridge and Extracellular Flux Analyzer Calibrant, Concanavalin A (ConA)-Coated Microplate, and Sperm Buffer Preparation
  • 01:52Cartridge Calibration Preparation and Wave Template Generation
  • 03:00Mouse Sperm Isolation
  • 03:42Sensor Cartridge Loading
  • 04:59Sperm Plate Preparation
  • 06:17Results: Representative Changes in Glycolysis and Oxidative Phosphorylation During Mouse Sperm Capacitation
  • 07:42Conclusion

Summary

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We describe the application of an extracellular flux analyzer to monitor real-time changes in glycolysis and oxidative phosphorylation during mouse sperm capacitation.

Tags

Extracellular Flux AnalyzerGlycolysisOxidative PhosphorylationMouse SpermCapacitationConATYH BufferWave TemplateSperm Metabolism

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