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通过组合单域抗体库的分步噬菌体选择创建高度特异性化学诱导蛋白分解系统
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生物化学
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JoVE 杂志 生物化学
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
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通过组合单域抗体库的分步噬菌体选择创建高度特异性化学诱导蛋白分解系统

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10:17 min

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January 14, 2020

DOI:

10.3791/60738-v

DOI:
10.3791/60738-v

•

10:17 min
January 14, 2020

•

4 Views
, , , ,
1Department of Biochemistry and Institute for Protein Design,University of Washington

Summary

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对于任何给定的小分子配体，创建具有所需亲和力和特异性的化学诱导蛋白二聚系统将具有许多生物传感和驱动应用。在这里，我们描述了一种有效的、可通用的方法，通过逐步选择噬菌体显示的组合单域抗体库来对化学诱导的二聚系统进行新工程。

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Cite this Article

Gomez-Castillo, L., Watanabe, K., Jiang, H., Kang, S., Gu, L. Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library. J. Vis. Exp. (155), e60738, doi:10.3791/60738 (2020).

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