Journal
/
生物学
/
체외에서 담즙 관내 형성에서 세포골격의 역할을 연구하기 위해 1 차 마우스 간세포의 격리 및 3D 콜라겐 샌드위치 문화
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
Please note that all translations are automatically generated. Click here for the English version.

체외에서 담즙 관내 형성에서 세포골격의 역할을 연구하기 위해 1 차 마우스 간세포의 격리 및 3D 콜라겐 샌드위치 문화

10,685 Views

•

10:12 min

•

December 20, 2019

DOI:

10.3791/60507-v

DOI:
10.3791/60507-v

•

10:12 min
December 20, 2019

•

9 Views
, , ,
1Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences

Tags

3D Collagen Sandwich CulturePrimary Mouse HepatocytesBile Canalicular FormationCytoskeletonLiver IsolationHepatocyte IsolationIn Vitro StudiesPerfusionHeparinDMEMCollagen IPBSCell Culture

Read Article

Cite this Article

Korelova, K., Jirouskova, M., Sarnova, L., Gregor, M. Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro. J. Vis. Exp. (154), e60507, doi:10.3791/60507 (2019).

Download .ris file

Copy

Share Video

.

Copy