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1Molecular Biology Interdepartmental Program,University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology,University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine,University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research,University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center,University of California, Los Angeles, 6Molecular Biology Institute,University of California, Los Angeles
Chapters
- 00:05Title
- 00:52Plating Sorted Prostate Epithelial Cells into Primary Mouse Organoid Culture
- 03:29Extracting Protein Lysate from Prostate Organoids for Western Blot Analysis
- 04:50Fixing and Staining Prostate Organoids for Immunohistochemical Analysis by Whole-Mount Confocal Microscopy
- 06:44Tissue Clearing and Mounting of the Stained Prostate Organoids for Whole-mount Confocal Microscopy
- 07:57Results: Analysis of Lineage Marker Expression in Prostate Organoids by Western Blot and Whole-Mount Confocal Microscopy
- 09:44Conclusion
Muis prostaat organoïden vertegenwoordigen een veelbelovende context voor het evalueren van mechanismen die differentiatie reguleren. Dit artikel beschrijft een verbeterde aanpak om prostaat organoïden vast te stellen, en introduceert methoden om (1) eiwit lysaat van organoïden te verzamelen, en (2) de organoïden voor de confocale microscopie van de hele Mount te fixeren en te vlechten.
