/

/

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

JoVE 杂志
遗传学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志遗传学

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

7,803 Views

•

09:10 min

•

July 09, 2019

DOI:

10.3791/59850-v

DOI:

10.3791/59850-v

•

09:10 min

July 09, 2019

•

7 Views

Wilson Pak-Kin Lou*^1,2, Liqun Wang*³, Cheng Long, Lei Liu, Ji-Feng Fei

¹School of Life Sciences,South China Normal University, ²The Research Institute of Molecular Pathology (IMP),Vienna Biocenter (VBC), ³Institute for Brain Research and Rehabilitation (IBRR),South China Normal University

Explore More Videos

Axolotl Spinal Cord Neural Stem Cells Gene Knock-out Electroporation CAS9 GRNA Regeneration Molecular Mechanisms Neural Stem Cell Function Spinal Cord Injury Agarose Electroporation Plate Poring Pulse Transfer Pulse

Read Article