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神经科学
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Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function

Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function

DOI:
10.3791/59680-v

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09:09 min

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August 07, 2019

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, , ,
1Institute of Pathophysiology,University Medical Center of the Johannes Gutenberg University Mainz

Chapters

  • 00:04Title
  • 00:38Dissection
  • 03:21Electrophysiology
  • 05:49Biocytin Labeling
  • 07:54Results: Morphological Analysis of a Relay Neuron
  • 08:29Conclusion

Summary

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Here, we present protocols for the preparation of acute brain slices containing the lateral geniculate nucleus and the electrophysiological investigation of retinogeniculate and corticogeniculate synapses function. This protocol provides an efficient way to study synapses with the high-release and low-release probability in the same acute brain slices.

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Keywords: ElectrophysiologicalRetinogeniculateCorticogeniculateSynapse FunctionLateral Geniculate NucleusRetinal Ganglion Cell AxonsRetinal Geniculate SynapsesCortical Geniculate SynapsesBrain SliceDissectionRecordingVibratomeForebrainCerebrumOlfactory BulbMidbrainHemispheresCutting StageBuffer Tray

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