需要订阅 JoVE 才能查看此. 登录或开始免费试用。
A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction
15,255 Views
•
00:05 min
•
August 27, 2019
DOI:
Summary
Automatically generated
In this study, we enhanced the data analysis capabilities of the DARTS experiment by monitoring the changes in protein stability and estimating the affinity of protein-ligand interactions. The interactions can be plotted into two curves: a proteolytic curve and a dose-dependence curve. We have used mTOR-rapamycin interaction as an exemplary case.
Read Article
Cite this Article
Zhang, C., Cui, M., Cui, Y., Hettinghouse, A., Liu, C. A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction. J. Vis. Exp. (150), e59656, doi:10.3791/59656 (2019).
Copy