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神经科学
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Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains

Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains

DOI:
10.3791/59568-v

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00:06 min

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August 26, 2019

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, , ,
1Nervous System Development and Homeostasis Laboratory,The Francis Crick Institute, 2Neuroscience Centre,Biomedical Sciences Research Centre “Al. Fleming”, 3Centre for Developmental Neurobiology,King’s College London

Chapters

  • 00:04Title
  • 00:41Mouse Embryo Cortical Slice Preparation
  • 02:27Focal DNA Injection
  • 03:53Acute Brain Slice Electroporation
  • 04:54Results: Representative Cortical Interneuron Progenitor Transplantation Analyses
  • 05:48Conclusion

Summary

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Here we present a protocol, designed to use chemogenetic tools to manipulate the activity of cortical interneuron progenitors transplanted into the cortex of early postnatal mice.

Tags

TransplantationChemogenetically EngineeredCortical Interneuron ProgenitorsPostnatal Mouse BrainCortical Interneuron PrecursorsCellular PropertiesCortical Interneuron ProgenitorsAgaroseVibrating Blade MicrotomeGanglionic EminencesFilter MembranesMinimal Essential MediumFocal DNA InjectionAgarose ColumnsElectroporation Electrode

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