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二次细菌性肺炎的鼠种模型的精确病原体输送与恢复系统
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
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二次细菌性肺炎的鼠种模型的精确病原体输送与恢复系统

5,302 Views

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00:13 min

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September 21, 2019

DOI:

10.3791/59566-v

DOI:
10.3791/59566-v

•

00:13 min
September 21, 2019

•

2 Views
, , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Summary

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在这里，我们提出的方法，以改善继发性细菌性肺炎研究，通过提供非侵入性途径注入下呼吸道，然后病原体恢复和抄本分析。这些程序是可重复的，无需专用设备（如导管、导丝或光纤电缆）即可执行。

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Secondary Bacterial PneumoniaMurine ModelPathogen DeliveryPathogen RecoveryTracheal InoculationBacterial CFU EnumerationRNA PurificationVirulence Gene AnalysisMinimally Invasive Technique

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Cite this Article

Borgogna, T. R., Sanchez-Gonzalez, A., Gorham, K., Voyich, J. M. A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia. J. Vis. Exp. (151), e59566, doi:10.3791/59566 (2019).

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