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Generation av 3-D kollagen-baserade Hydrogels att analysera Axonal tillväxt och beteende under nerv systemet utveckling
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development
DOI:

09:10 min

June 25, 2019

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Chapters

  • 00:04Title
  • 00:36Preparation of Cell (COS1) Aggregates Genetically-modified to Secrete a Candidate Molecule in 3-D collagen Hydrogels
  • 04:03Generation of Embryonic Explant for Culture
  • 05:54Preparation of 3-D Co-cultures in Collagen Hydrogels
  • 07:25Results: Analysis of Axonal Growth and Behavior
  • 08:39Conclusion

Summary

自动翻译

Här ger vi en metod för att analysera beteendet hos växande axoner i 3D-matriser, imitera deras naturliga utveckling.

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