需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity
5,990 Views
•
08:00 min
•
July 10, 2019
DOI:
1Department of Biomedical Engineering, School of Medicine, School of Engineering,University of Alabama at Birmingham, 2Division of Cardiovascular Diseases, School of Medicine, School of Engineering,University of Alabama at Birmingham, 3Department of Cardiac Surgery,The Second Xiangya Hospital of Central South University
Summary
Automatically generated
In this protocol, we demonstrate and elaborate on how to use human induced pluripotent stem cells for cardiomyocyte differentiation and purification, and further, on how to improve its transplantation efficiency with Rho-associated protein kinase inhibitor pretreatment in a mouse myocardial infarction model.
Read Article
Cite this Article
Zhao, M., Tang, Y., Ernst, P. J., Kahn-Krell, A., Fan, C., Pretorius, D., Zhu, H., Lou, X., Zhou, L., Zhang, J., Zhu, W. Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity. J. Vis. Exp. (149), e59452, doi:10.3791/59452 (2019).
Copy