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A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

DOI:
10.3791/59333-v

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00:12 min

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September 22, 2019

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, , ,
1National Institute of Neurological Disorders and Stroke (NINDS), Neurotherapeutic Development Unit (NTDU),National Institutes of Health (NIH)

Chapters

  • 00:04Title
  • 00:58Dissociate and Plate Neural Stem Cells
  • 03:12Treating Cells with Compounds
  • 06:10Imaging Cells on a Plate Reader
  • 08:27Terminal MTT (3-(4,5-dimethylthizol-2-yl)-2,5-diphenyltetrazolium bromide) Cytotoxicity Assay
  • 10:04Results: tdTomato Assay and MTT Assay
  • 11:40Conclusion

Summary

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It is often necessary to assess the potential cytotoxicity of a set of compounds on cultured cells. Here, we describe a strategy to reliably screen for toxic compounds in a 96-well format.

Tags

Cell GrowthCell SurvivalCell CultureCompound ScreeningCytotoxicityCell ProliferationCell ViabilityHigh-throughputLow-to-moderate ThroughputMulti-assay StrategyCell DissociationCell Counting96-well PlateExtracellular Matrix CoatingCell Culture Incubation

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