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Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors
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神经科学
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JoVE 杂志 神经科学
Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

DOI:
10.3791/59321-v

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07:33 min

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May 01, 2019

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, , , , , , ,
1Department of Biology and Biotechnology Charles Darwin,Sapienza University of Rome, Italy, 2Center for Life Nano Science,Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology,The Rockefeller University, USA, 4Department of Physiology and Pharmacology,Sapienza University of Rome, Italy

Chapters

  • 00:04Title
  • 00:54Ngn2, Isl1 and Lhx3 (NIL) and Ngn2, Isl1 and Phox2a (NIP) Induced Pluripotent Stem Cell (iPSC) Line Generation
  • 02:19Antibiotic Selection
  • 03:18Motor Neuron Differentiation
  • 05:12Results: Representative Human iPSC Motor Neuron (MN) Differentiation Analysis
  • 07:01Conclusion

Summary

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自动翻译

This protocol allows rapid and efficient conversion of induced pluripotent stem cells into motor neurons with a spinal or cranial identity, by ectopic expression of transcription factors from inducible piggyBac vectors.

Tags

Human Induced Pluripotent Stem CellsIPSCsSpinal Motor NeuronsCranial Motor NeuronsPiggyBac VectorsMotor Neuron DiseasesAmyotrophic Lateral SclerosisCell DifferentiationTransfectionBlasticidin SelectionDoxycycline InductionRT-PCR

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