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发育生物学
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Citometría de flujo en tiempo real de células vivas para investigar la afluencia del calcio, formación de poro y fagocitosis por los receptores P2X7 en células progenitoras neurales adultas
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
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Citometría de flujo en tiempo real de células vivas para investigar la afluencia del calcio, formación de poro y fagocitosis por los receptores P2X7 en células progenitoras neurales adultas

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11:47 min

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April 03, 2019

DOI:

10.3791/59313-v

DOI:
10.3791/59313-v

•

11:47 min
April 03, 2019

•

1 Views
, , , , ,
1Griffith Institute for Drug Discovery,Griffith University, 2Australian Institute for Bioengineering and Nanotechnology,University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science,University of Sydney, 4Bosch Institute,University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit,St. Vincent’s Centre for Applied Medical Research, 6School of Medical Sciences,The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science,Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health,University of Melbourne

Summary

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Proporcionar sensibilidad unicelular, citometría de flujo en tiempo real está especialmente preparado para cuantificar las funciones de receptor multimodal de cultivos vivos. Con células progenitoras neurales adultas, la función del receptor P2X7 se evaluó mediante la afluencia de calcio detectado por colorante indicador de calcio, formación de poros transmembrana por absorción de bromuro de etidio y fagocitosis usando gotas de látex fluorescentes.

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Cite this Article

Leeson, H. C., Chan-Ling, T., Lovelace, M. D., Brownlie, J. D., Weible II, M. W., Gu, B. J. Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells. J. Vis. Exp. (146), e59313, doi:10.3791/59313 (2019).

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