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Real-Time Live-cel Stroom Cytometry te onderzoeken Calcium toestroom, porie vorming en fagocytose door P2X7 receptoren in volwassen neurale voorlopercellen
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
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Real-Time Live-cel Stroom Cytometry te onderzoeken Calcium toestroom, porie vorming en fagocytose door P2X7 receptoren in volwassen neurale voorlopercellen

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11:47 min

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April 03, 2019

DOI:

10.3791/59313-v

DOI:
10.3791/59313-v

•

11:47 min
April 03, 2019

•

1 Views
, , , , ,
1Griffith Institute for Drug Discovery,Griffith University, 2Australian Institute for Bioengineering and Nanotechnology,University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science,University of Sydney, 4Bosch Institute,University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit,St. Vincent’s Centre for Applied Medical Research, 6School of Medical Sciences,The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science,Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health,University of Melbourne

Summary

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Verstrekken van eencellige gevoeligheid, is real-time stroom cytometry uniek geschikt voor het kwantificeren van multimodale receptor functies van levende culturen. Met behulp van neurale volwassen voorlopercellen, de P2X7 receptor functie evalueerden via calcium toestroom gedetecteerd door calcium indicator kleurstof, transmembraan porie vorming door ethidium bromide opname en fagocytose met behulp van fluorescerende latex kralen.

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Leeson, H. C., Chan-Ling, T., Lovelace, M. D., Brownlie, J. D., Weible II, M. W., Gu, B. J. Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells. J. Vis. Exp. (146), e59313, doi:10.3791/59313 (2019).

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