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En alsidig metode til montering Arabidopsis blade til Intravital Time-lapse Imaging
JoVE 杂志
生物学
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JoVE 杂志 生物学
A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging
DOI:

07:52 min

February 11, 2019

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Chapters

  • 00:04Title
  • 00:28Preparation of Plants and Pathogens
  • 01:48Pathogen Inoculation
  • 03:10Mounting the Inoculated Leaf
  • 05:02Microscopic Time-lapse Observation
  • 06:19Results: Intravital Time-lapse Imaging of Mounted Arabidopsis Leaves
  • 07:16Conclusion

Summary

自动翻译

Vi rapporterer en simpel og alsidig metode til at udføre fluorescerende live-billeddannelse af Arabidopsis thaliana blade over en længere periode. Vi bruger en gensplejsede Arabidopsis plante at udtrykke et fluorescerende reporter gen under kontrol af en immunitet-relaterede promotor som et eksempel for at få spatiotemporelle forståelse af plante immunrespons.

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