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一种用于阴南芥叶片安装的通用方法
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging
DOI:

07:52 min

February 11, 2019

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Chapters

  • 00:04Title
  • 00:28Preparation of Plants and Pathogens
  • 01:48Pathogen Inoculation
  • 03:10Mounting the Inoculated Leaf
  • 05:02Microscopic Time-lapse Observation
  • 06:19Results: Intravital Time-lapse Imaging of Mounted Arabidopsis Leaves
  • 07:16Conclusion

Summary

自动翻译

我们报告了一种简单和通用的方法, 用于在较长时间内对拟南芥叶片进行荧光实时成像。我们使用转基因拟南芥植物表达荧光记者基因在免疫相关的启动子控制下作为一个例子, 以获得时空了解植物免疫反应。

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