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Siderofoor High-throughput Screening van milieu monsters: plantaardige weefsels, Bulk bodems en rhizosfeer bodems
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
DOI:

12:36 min

February 09, 2019

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Chapters

  • 00:04Title
  • 01:01Preparation of Chrome Azurol S (CAS)-Fe-Agar Medium
  • 03:35Pyoverdine/EDTA Standard Preparation
  • 05:04Collection of Environmental Samples: Soil and Plant Tissues
  • 06:38Preparation of Siderophore Enrichment Cultures and CAS-Fe Siderophore Production Assay
  • 08:11CAS-Fe Agar Assays for Detection of Siderophore Production in Environmental Samples
  • 09:34Results: Pyoverdine Equivalents of Siderophore Enrichment Cultures
  • 11:28Conclusion

Summary

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Presenteren we een protocol voor snelle screening van milieu monsters voor siderofoor potentieel bijdragen aan micronutriënt biologische beschikbaarheid en omzet in terrestrische systemen.

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