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Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy
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神经科学
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JoVE 杂志 神经科学
Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy

Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy

DOI:
10.3791/59036-v

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07:02 min

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January 19, 2019

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, , ,
1Department of Neurology,University of Michigan School of Medicine, 2Neuroscience Graduate Program,University of Michigan School of Medicine

Chapters

  • 00:04Title
  • 01:06Transfection of Rat Cortical Neurons
  • 02:22Imaging
  • 03:09Image Processing and Scoring Cell Death
  • 04:35Performing Cox Proportional Hazards Analysis and Visualizing Results
  • 05:19Results: Assessing Neuronal Survival on a Single-cell Basis
  • 06:16Conclusion

Summary

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Here, we present a protocol to monitor survival on a single-cell basis and identify variables that significantly predict cell death.

Tags

Keywords: Neuronal SurvivalLongitudinal Fluorescence MicroscopySingle-cell MonitoringCytotoxicity AssaysNeurodegenerative DiseasesCell DeathTransfection EfficiencyMultichannel PipetCortical NeuronsNBKY SolutionConditioned MediaNBC SolutionFluorescent MicroscopeFiduciary MarkX And Y CoordinatesFluorescent Label

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