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生物大分子晶体的完全自主表征及数据采集
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
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生物大分子晶体的完全自主表征及数据采集

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07:11 min

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March 22, 2019

DOI:

10.3791/59032-v

DOI:
10.3791/59032-v

•

07:11 min
March 22, 2019

•

1 Views
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1Structural Biology Group,European Synchrotron Radiation Facility, 2Grenoble Outstation,European Molecular Biology Laboratory

Summary

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在这里, 我们将介绍如何使用某些同步加速器波束线提供的自动筛选和数据收集选项。科学家将冷冻样品发送到同步加速器, 并对衍射特性进行筛选, 收集和处理数据集, 并在可能的情况下执行结构解决方案--所有这些都无需人工干预。

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Cite this Article

Hutin, S., Van Laer, B., Mueller-Dieckmann, C., Leonard, G., Nurizzo, D., Bowler, M. W. Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules. J. Vis. Exp. (145), e59032, doi:10.3791/59032 (2019).

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