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Genetische manipulatie van Dictyostelium discoideum cellen op basis van de selectie en de groei van bacteriën
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
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Genetische manipulatie van Dictyostelium discoideum cellen op basis van de selectie en de groei van bacteriën

12,344 Views

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06:08 min

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January 25, 2019

DOI:

10.3791/58981-v

DOI:
10.3791/58981-v

•

06:08 min
January 25, 2019

•

11 Views
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1MRC Laboratory of Molecular Biology, 2Department of Molecular and Cell Biology,University of Connecticut, 3Cancer Research UK Beatson Institute Glasgow, 4MRC Laboratory for Molecular Cell Biology,University College London, 5Department of Cell and Developmental Biology,University College London

Summary

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Dictyostelium-discoideum is een populair model-organisme te bestuderen van complexe cellulaire processen zoals cel migratie, endocytose en ontwikkeling. Het nut van het organisme is afhankelijk van de haalbaarheid van genetische manipulatie. Hier presenteren we methoden om te transfect Dictyostelium discoideum cellen die bestaande van kweken cellen in vloeibare media beperkingen.

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Paschke, P., Knecht, D. A., Williams, T. D., Thomason, P. A., Insall, R. H., Chubb, J. R., Kay, R. R., Veltman, D. M. Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria. J. Vis. Exp. (143), e58981, doi:10.3791/58981 (2019).

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