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Ingegneria genetica di Dictyostelium discoideum cellule basata sulla selezione e crescita sui batteri
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遗传学
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JoVE 杂志 遗传学
Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
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Ingegneria genetica di Dictyostelium discoideum cellule basata sulla selezione e crescita sui batteri

12,344 Views

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06:08 min

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January 25, 2019

DOI:

10.3791/58981-v

DOI:
10.3791/58981-v

•

06:08 min
January 25, 2019

•

11 Views
, , , , , , ,
1MRC Laboratory of Molecular Biology, 2Department of Molecular and Cell Biology,University of Connecticut, 3Cancer Research UK Beatson Institute Glasgow, 4MRC Laboratory for Molecular Cell Biology,University College London, 5Department of Cell and Developmental Biology,University College London

Summary

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Dictyostelium discoideum è un organismo modello popolare per studiare i complessi processi cellulari come la migrazione cellulare, endocitosi e sviluppo. L'utilità dell'organismo è dipenda sulla fattibilità di manipolazione genetica. Qui, presentiamo metodi per transfect Dictyostelium discoideum cellule che superare i limiti esistenti delle cellule di coltura in terreno liquido.

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Paschke, P., Knecht, D. A., Williams, T. D., Thomason, P. A., Insall, R. H., Chubb, J. R., Kay, R. R., Veltman, D. M. Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria. J. Vis. Exp. (143), e58981, doi:10.3791/58981 (2019).

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