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基于细菌选择和生长的盘状细胞基因工程

JoVE 杂志
遗传学

This content is Free Access.

JoVE 杂志遗传学

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

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基于细菌选择和生长的盘状细胞基因工程

12,344 Views

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06:08 min

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January 25, 2019

DOI:

10.3791/58981-v

DOI:

10.3791/58981-v

•

06:08 min

January 25, 2019

•

11 Views

Peggy Paschke¹, David A. Knecht², Thomas D. Williams¹, Peter A. Thomason³, Robert H. Insall³, Jonathan R. Chubb^4,5, Robert R. Kay¹, Douwe M. Veltman¹

¹MRC Laboratory of Molecular Biology, ²Department of Molecular and Cell Biology,University of Connecticut, ³Cancer Research UK Beatson Institute Glasgow, ⁴MRC Laboratory for Molecular Cell Biology,University College London, ⁵Department of Cell and Developmental Biology,University College London

Summary

盘状双翅目是研究细胞迁移、内吞和发育等复杂细胞过程的常用模型生物。生物体的效用取决于基因操纵的可行性。在这里, 我们提出了方法, 以转移牙菌细胞, 克服现有的限制培养细胞在液体介质。

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