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通过Sialoglycan的代谢标记识别主要神经干细胞和祖细胞的细胞表面标记
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
DOI:

11:39 min

September 07, 2019

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Chapters

  • 00:05Title
  • 01:16Preparation of Mouse Endothelial Culture in Permeable Support Inserts
  • 01:55Set-up of Neural-endothelial Co-culture and Ac4ManNAz Labeling System
  • 03:25Immunofluorescent Staining of Sialoglycoproteins in Expanded Primary NSPCs and Differentiated Neurons
  • 05:25Purification of Sialoglycoproteins from Expanded Primary NSPCs and Differentiated Neurons
  • 08:45Results: Identifying Cell Surface Markers by Metabolic Labeling of Sialoglycan
  • 10:47Conclusion

Summary

自动翻译

这里介绍的是一个协议,结合了体外神经内皮共培养系统和代谢结合的异形理念功能组,以扩大主神经干和祖细胞,并标记其表面用于细胞表面标记的成像或质谱分析的紫锥蛋白。

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