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crispr 介导的人类真菌病原体白色念珠菌基因组编辑
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
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crispr 介导的人类真菌病原体白色念珠菌基因组编辑

11,321 Views

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09:56 min

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November 14, 2018

DOI:

10.3791/58764-v

DOI:
10.3791/58764-v

•

09:56 min
November 14, 2018

•

4 Views
, , ,
1Department of Biology,Ball State University, 2Whitehead Institute for Biomedical Research

Summary

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白色念珠菌的高效基因组工程是了解治疗方法的发病机制和发展的关键。在这里, 我们描述了一个协议, 以快速和准确地编辑白色念珠菌基因组使用 crispr. 该协议允许调查人员引入各种各样的基因修饰, 包括点突变、插入和删除。

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Cite this Article

Evans, B. A., Pickerill, E. S., Vyas, V. K., Bernstein, D. A. CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans. J. Vis. Exp. (141), e58764, doi:10.3791/58764 (2018).

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