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Hög känslighet mätning av transkription faktorn-DNA bindande samhörighet genom konkurrenskraftiga titrering med fluorescensmikroskopi
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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Hög känslighet mätning av transkription faktorn-DNA bindande samhörighet genom konkurrenskraftiga titrering med fluorescensmikroskopi

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06:38 min

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February 07, 2019

DOI:

10.3791/58763-v

DOI:
10.3791/58763-v

•

06:38 min
February 07, 2019

•

2 Views
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1Gene Center and Department of Biochemistry, Center for Protein Science Munich (CIPSM),Ludwig-Maximilians-Universität München

Tags

Transcription Factor-DNA BindingFluorescence MicroscopyDissociation ConstantCompetitive TitrationAnisotropy MeasurementBinding AffinityProtein-DNA InteractionDNA AnnealingAgarose GelTitration PlateCalibration WellNile Blue Dye

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Jung, C., Schnepf, M., Bandilla, P., Unnerstall, U., Gaul, U. High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy. J. Vis. Exp. (144), e58763, doi:10.3791/58763 (2019).

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