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Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
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JoVE 杂志 免疫与感染
Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

5,829 Views

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06:54 min

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June 15, 2019

DOI:

10.3791/58688-v

DOI:
10.3791/58688-v

•

06:54 min
June 15, 2019

•

11 Views
, , , , , , , ,
1Department of Life Sciences,University of Trieste, 2Institute for Maternal and Child Health,IRCCS (Istituto di Ricovero e Cura a Carattere Scientifico) Burlo Garofolo, 3Department of Medical, Surgical and Health Science,University of Trieste

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The complement component C1q is a pro-inflammatory molecule highly expressed in the tissue microenvironment that can interact with the extracellular matrix. Here, we describe a method to test how C1q bound to hyaluronic acid impacts cell adhesion.

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Vidergar, R., Agostinis, C., Zacchi, P., Mangogna, A., Bossi, F., Zanconati, F., Confalonieri, M., Ricci, G., Bulla, R. Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion. J. Vis. Exp. (148), e58688, doi:10.3791/58688 (2019).

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