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Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model
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JoVE 杂志 免疫与感染
Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model

Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model

DOI:
10.3791/58668-v

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09:31 min

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November 09, 2018

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, , , , , , , ,
1College of Life Sciences,Zhejiang University, 2Monell Chemical Senses Center

Chapters

  • 00:04Title
  • 01:20Dextran Sulfate Sodium (DSS) Colitis Induction and Evaluation
  • 02:49Histological Assessement
  • 04:48Immunohistochemistry and Gene Expression Assessment
  • 07:19Results: Representative Effects of Taste Signaling Protein Ablation on Gut Inflammation in DSS-induced Mice
  • 08:23Conclusion

Summary

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Here we present a protocol to investigate the effect of the nullification of gustation-related genes on immune responses in a dextran sulfate sodium (DSS)-induced inflammatory bowel disease (IBD) mouse model.

Tags

Inflammatory Bowel DiseaseUlcerative ColitisCrohn’s DiseaseDextran Sulfate SodiumColonHistologyHematoxylin And Eosin StainingCryoprotection

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