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Localization of the Locus Coeruleus in the Mouse Brain
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神经科学
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JoVE 杂志 神经科学
Localization of the Locus Coeruleus in the Mouse Brain

Localization of the Locus Coeruleus in the Mouse Brain

DOI:
10.3791/58652-v

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07:44 min

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March 07, 2019

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, , , , , ,
1Department of Physiology,Johns Hopkins University, School of Medicine, 2Department of Neuroscience,Johns Hopkins University, 3Department of Molecular and Medical Genetics,OHSU, 4X-ray science division, Advanced Photon Source,Argonne National Laboratory

Chapters

  • 00:04Title
  • 00:46Brain Slicing
  • 03:23Immunohistochemistry for Detection of the Locus Coeruleus in Brain Slices
  • 05:34Results: Detection of Proteins Expressed in LC and Changes in Metal Homeostasis in Brain Sections
  • 06:24Conclusion

Summary

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The locus coeruleus is a small cluster of neurons involved in a variety of physiological processes. Here, we describe a protocol to prepare mouse brain sections for studies of proteins and metals in this nucleus.

Tags

Locus CoeruleusMouse BrainLocalizationNeurological DisordersBrain SectioningBrain EmbeddingCryostatBrain OrientationBrainstemCerebellumFourth Ventricle

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