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Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

6,001 Views

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07:25 min

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March 20, 2019

DOI:

10.3791/58524-v

DOI:
10.3791/58524-v

•

07:25 min
March 20, 2019

•

4 Views
,
1Instituto Agroalimentario de Aragón – IA2 (CITA-Universidad de Zaragoza),Centro de Investigación y Tecnología Agroalimentaria de Aragón

Summary

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We present a methodology to quantify the starch content in the ovary primordia in sweet cherry (Prunus avium L.) during winter dormancy by using an image analysis system combined with histochemical techniques.

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Starch QuantificationOvary PrimordiaSweet CherryWinter DormancyHistochemical StainingImage AnalysisFlower BudsDormancyReproductive PhaseLugol’s Iodine StainingBrightfield MicroscopyImage Acquisition

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Cite this Article

Fadon, E., Rodrigo, J. Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy. J. Vis. Exp. (145), e58524, doi:10.3791/58524 (2019).

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