We describe the methods for fluorescent labeling of tangentially migrating cells by electroporation, and for time-lapse imaging of the labeled cell movement in a flat-mount culture in order to visualize migrating cell behavior in the developing chick optic tectum.
Watanabe, Y., Sakuma, C., Yaginuma, H. Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum. J. Vis. Exp. (140), e58506, doi:10.3791/58506 (2018).