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Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

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08:12 min

September 28, 2018

DOI:

08:12 min
September 28, 2018

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Summary

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A protocol for the elucidation of plant thylakoid protein complex organization and composition with blue native polyacrylamide gel electrophoresis (BN-PAGE) and 2D-SDS-PAGE is described. The protocol is optimized for Arabidopsis thaliana, but can be used for other plant species with minor modifications.

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