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Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

DOI:
10.3791/58347-v

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08:42 min

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October 04, 2018

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, ,
1Department of Physiology and Cell Biology, School of Medicine,University of Nevada, 2Department of Pharmacology, Larner College of Medicine,University of Vermont

Chapters

  • 00:04Title
  • 00:27Preparation of the Diaphragms and Phrenic Nerves from Transgenic Mice
  • 01:27Stimulation and Recording of the Muscle Action Potentials
  • 04:49Imaging of the Fluorescence of the Sample
  • 06:33Results: Nerve Stimulation-induced Ca2+ Responses in Populations of Schwann Cells or Muscle Cells of the Neonatal Mouse Diaphragm
  • 08:03Conclusion

Summary

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自动翻译

Here we present a protocol to image calcium signaling in populations of individual cell types at the murine neuromuscular junction.

Tags

Ex Vivo ImagingCalcium SignalingTripartite SynapseNeuromuscularPhrenic NerveDiaphragmNerve StimulationMuscle CellsSchwann CellsTransgenic MiceKrebs-Ringer SolutionAlpha BungarotoxinSuction ElectrodeBHCMu-conotoxin

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