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Microgravimetry dissipativo para estudar a dinâmica da vinculação do fosfolipídeo proteína anexina A2 para Bilayers do Lipid com suporte sólido usando um ressonador de quartzo
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Microgravimetry dissipativo para estudar a dinâmica da vinculação do fosfolipídeo proteína anexina A2 para Bilayers do Lipid com suporte sólido usando um ressonador de quartzo

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07:11 min

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November 01, 2018

DOI:

10.3791/58224-v

DOI:
10.3791/58224-v

•

07:11 min
November 01, 2018

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6 Views
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1Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation,University of Münster, 2Institute of Biochemistry,University of Münster, 3Cluster of Excellence ‘Cells in Motion’,University of Münster

Tags

Dissipative MicrogravimetryPhospholipid Binding ProteinAnnexin A2Solid-supported Lipid BilayersQuartz ResonatorCalcium-dependent BindingNegatively Charged PhospholipidsLabel-freeSensitiveQuantitativeReal-time AnalysisLipid SolutionsMultilamellar VesiclesSmall Unilamellar VesiclesSDS CleaningPlasma Cleaning

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Matos, A. L. L., Grill, D., Kudruk, S., Heitzig, N., Galla, H., Gerke, V., Rescher, U. Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator. J. Vis. Exp. (141), e58224, doi:10.3791/58224 (2018).

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