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用石英谐振器研究磷脂结合蛋白附件 a2 与固体支持的脂质层结合动力学的耗散微重法
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
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用石英谐振器研究磷脂结合蛋白附件 a2 与固体支持的脂质层结合动力学的耗散微重法

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07:11 min

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November 01, 2018

DOI:

10.3791/58224-v

DOI:
10.3791/58224-v

•

07:11 min
November 01, 2018

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6 Views
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1Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation,University of Münster, 2Institute of Biochemistry,University of Münster, 3Cluster of Excellence ‘Cells in Motion’,University of Münster

Tags

Dissipative MicrogravimetryPhospholipid Binding ProteinAnnexin A2Solid-supported Lipid BilayersQuartz ResonatorCalcium-dependent BindingNegatively Charged PhospholipidsLabel-freeSensitiveQuantitativeReal-time AnalysisLipid SolutionsMultilamellar VesiclesSmall Unilamellar VesiclesSDS CleaningPlasma Cleaning

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Matos, A. L. L., Grill, D., Kudruk, S., Heitzig, N., Galla, H., Gerke, V., Rescher, U. Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator. J. Vis. Exp. (141), e58224, doi:10.3791/58224 (2018).

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