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Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

DOI:
10.3791/58152-v

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09:36 min

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August 29, 2018

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, , , ,
1Hotchkiss Brain Institute, Department of Cell Biology and Anatomy, Cumming School of Medicine,University of Calgary, 2Department of Developmental and Stem Cell Biology Program, Arthur and Sonia Labatt Brain Tumor Research Centre,The Hospital for Sick Children

Chapters

  • 00:04Title
  • 00:48Human Glioblastoma Specimen-derived Brain Tumor Stem Cell (BTSC) Culture
  • 02:40BTSC Migration Assay
  • 05:01BTSC Neurosphere Invasion Assay
  • 07:16Results: Representative BTSC Migration and Invasion Analyses
  • 09:00Conclusion

Summary

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Here, we describe live-cell imaging techniques to quantitatively measure the migration and invasion of glioblastoma brain tumor stem cells over time and under multiple treatment conditions.

Tags

Live-cell ImagingGlioblastomaBrain Tumor Stem Cells (BTSCs)MigrationInvasionCancer Stem CellsCell CultureNeurosphereCell DetachmentChemotaxisDrug Treatment

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