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体外犬中性粒细胞胞外陷阱的形成: 荧光显微镜动态定量分析
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In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
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体外犬中性粒细胞胞外陷阱的形成: 荧光显微镜动态定量分析

8,509 Views

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09:45 min

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August 24, 2018

DOI:

10.3791/58083-v

DOI:
10.3791/58083-v

•

09:45 min
August 24, 2018

•

4 Views
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1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine,University of California, Davis

Summary

Automatically generated

我们描述了从全血中分离犬中性粒细胞的方法, 用荧光显微镜观察活中性粒细胞的网状形成。还描述了使用免疫荧光显微镜定量分析网络形成和瓜组蛋白 H3 (citH3) 表达的协议。

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Li, R. H., Tablin, F. In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy. J. Vis. Exp. (138), e58083, doi:10.3791/58083 (2018).

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