Journal
/
/
La identificación de las feromonas de la lamprea de mar usando fraccionamiento guiado por bioensayo
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
DOI:

09:35 min

July 17, 2018

, ,

Chapters

  • 00:04Title
  • 00:27Collection and Extraction of Sea Lamprey Conditioned Water
  • 01:14Isolation of Fraction Pools with Chromatography
  • 02:51Electro-olfactogram Recordings to Identify Odorous Fractions/Compounds
  • 05:43Two-choice Maze Behavioral Bioassay to Identify Behaviorally Active Fractions/Compounds
  • 06:31Chromatographic Isolation of Pure Compounds from Active Fractions
  • 06:58Structure Elucidation of a Pure Compound with Mass Spectrometry and Nuclear Magnetic Resonance
  • 07:58Results: Behavioral Responses of Ovulated Female Sea Lampreys to Odorants
  • 08:36Conclusion

Summary

自动翻译

Aquí, presentamos un protocolo para aislar y caracterizar la estructura, potencia olfativa y respuesta comportamiento de compuestos de feromonas putativos de lampreas de mar.

Related Videos

Read Article