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甲苯胺蓝染色树脂嵌段评价周围神经形态学
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology
DOI:

08:17 min

July 03, 2018

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Chapters

  • 00:04Title
  • 00:36Surgery and In Vivo Nerve Fixation
  • 02:32Osmium Tetroxide Treatment and Resin Embedding
  • 04:38Sectioning by Ultramicrotome
  • 05:58Toluidine Blue Staining
  • 06:47Results: Transverse Section Images of a Rat Sciatic Nerve Stained with Toluidine Blue
  • 07:48Conclusion

Summary

自动翻译

在这里, 我们提出了一个通过获取和染色1-2 µm 段与甲苯胺蓝可视化周围神经精细结构的协议

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