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従来のひと多能性幹細胞の向上多能分化と素朴なような状態の復帰化学
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发育生物学
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JoVE 杂志 发育生物学
Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
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従来のひと多能性幹細胞の向上多能分化と素朴なような状態の復帰化学

9,727 Views

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09:07 min

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June 10, 2018

DOI:

10.3791/57921-v

DOI:
10.3791/57921-v

•

09:07 min
June 10, 2018

•

4 Views
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1Department of Oncology, Division of Pediatric Oncology and Institute for Cell Engineering,Johns Hopkins School of Medicine

Summary

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効率的な一括、および急速な化学復帰従来系列プライミングひと多能性幹細胞 (hPSC) の epigenomically 安定した素朴な着床前胚盤葉上層のような多能性状態にするためのプロトコルを提案します。このメソッド結果減少系統プライミング遺伝子発現と分化多能でマーク付きの改善で従来の hPSC ラインの幅広いレパートリー。

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Chemical ReversionConventional Human Pluripotent Stem CellsNaive-like StateMultilineage DifferentiationLIF-3i MethodPre-implantation EpiblastLineage-primed Gene ExpressionEpigenomic StabilityRegenerative MedicineHPSC DifferentiationGene TargetingInterspecies ChimerasLIF-5i MediumCell DissociationFeeder-free Culture

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Cite this Article

Park, T. S., Zimmerlin, L., Evans-Moses, R., Zambidis, E. T. Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency. J. Vis. Exp. (136), e57921, doi:10.3791/57921 (2018).

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