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In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

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05:52 min

June 28, 2018

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05:52 min
June 28, 2018

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Summary

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Bioluminescent bacteria regulate light production through a variety of mechanisms, such as quorum sensing. This novel method allows the in situ investigation of bioluminescence and the correlation of light emission to cell density. An artificial bioluminescent Escherichia coli system allows the characterization of lux operons, Lux proteins, and their interplay.

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