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Definiera Hsp33's Redox-reglerade förkläde aktivitet och mappning konfirmerande ändringar på Hsp33 med hjälp av väte-deuterium Exchange masspektrometri
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生物化学
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Defining Hsp33’s Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
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Definiera Hsp33's Redox-reglerade förkläde aktivitet och mappning konfirmerande ändringar på Hsp33 med hjälp av väte-deuterium Exchange masspektrometri

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10:24 min

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June 07, 2018

DOI:

10.3791/57806-v

DOI:
10.3791/57806-v

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10:24 min
June 07, 2018

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2 Views
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1Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram,The Hebrew University of Jerusalem

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Hsp33Chaperone ActivityHydrogen-deuterium ExchangeMass SpectrometryOxidative StressProtein AggregationProtein DynamicsEnzyme InteractionsVisual Demonstration

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Fassler, R., Edinger, N., Rimon, O., Reichmann, D. Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry. J. Vis. Exp. (136), e57806, doi:10.3791/57806 (2018).

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